BbsI from New England Biolabs

Product Specs
ItemBbsI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0539S
Quantity300 units
Concentration5 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAAGAC(2/6)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nutlin-3a induces KRAS mutant/p53 wild type lung cancer specific methuosis-like cell death that is dependent on GFPT2J Exp Clin Cancer ResDecember 14, 2023Dasom Kim, Dongwha Min, Joohee Kim, Min Jung Kim, Yerim Seo, Byung Hwa Jung, Seung-Hae Kwon, Hyunju Ro, Seoee Lee, Jason K. Sa, et al.The pX330 plasmid, purified PCR product, ATP, T4 DNA ligase, and BbsI enzyme (New England Biolabs, R0539S) were combined in vitro for 1 h at 37 °C, and then transformed into the DH5α for the amplification of

(2) Spatiotemporal genomic profiling of intestinal metaplasia reveals clonal dynamics of gastric cancer progressionCancer CellDecember 11, 2023Kie Kyon Huang, Haoran Ma, Roxanne Hui Heng Chong, Tomoyuki Uchihara, Benedict Shi Xiang Lian, Feng Zhu, Taotao Sheng, Supriya Srivastava, Su Ting Tay, Raghav Sundar, et al.Pack,Nanostring,GMX-NGS-SEQ-AB #121400201 T4 Polynucleotide Kinase,New England Biolabs,T M02015 BbsI,New England Biolabs,R0539S BsaI-HF,New England Biolabs,R3733S RNeasy Mini Kit,Qiagen,74106 GEL/PCR Purification Mini Kit,Favorgen,FAGCK

(3) Protocol for the saturation and multiplexing of genetic variants using CRISPR-Cas9STAR ProtocolsDecember 1, 2023Sounak Sahu, Teresa Sullivan, Eileen Southon, Dylan Caylor, Josephine Geh, Shyam K. SharanT4 polynucleotide kinase enzyme,New England Biolabs,Cat# M0201S BbsI (high fidelity) enzyme,New England Biolabs,Cat# R0539S T7 DNA ligase enzyme,New England Biolabs,Cat# M0318S ,, Recombinant DNA,Recombinant DNA,Recombinant

(4) CHIP protects against septic acute kidney injury by inhibiting NLRP3-mediated pyroptosisiScienceOctober 20, 2023Hao Zhang, Zebin Deng, Yilong Wang, Xiaoping Zheng, Lizhi Zhou, Shu Yan, Yinhuai Wang, Yingbo Dai, Yashpal.S. Kanwar, Fei DengCRISPR/Cas9 system was used in our studies. pSpCas9(BB)-2A-Puro (PX459) plasmid was cut by BbsI (NEB, #R0539S), and the reaction mixture was purified with gel extraction. SgRNA was designed with the online website:

(5) The enhancement of CRISPR/Cas9 gene editing using metforminBiochemistry and Biophysics ReportsSeptember 10, 2023Jaedyn L. Rollins, Raquel M. Hall, Clara J. Lemus, Lauren A. Leisten, Jennifer M. Johnstonwas incorporated into pX330 downstream of the U6 promoter via BbsI digestion (New England Biolabs #R0539L) using T4 DNA Ligase (New England Biolabs #M0202L). A total of five PF4 and four RhD guide RNA expression

(6) The effect of histone deacetylase inhibitors on the efficiency of the CRISPR/Cas9 systemBiochemistry and Biophysics ReportsSeptember 1, 2023Ymer Björnson, Codey Y. Huang, Jaedyn L. Rollins, Guadalupe Castañeda, Navneet Kaur, Emiko Yamamoto, Jennifer M. Johnstonphosphorylated using PNK (New England Biolabs #M0201L). They were then ligated into a BbsI (New England Biolabs #R0539L) digested pX330 plasmid using T4 DNA Ligase (New England Biolabs #M0202L). Guide integration was confirmed

(7) Gastruloid-derived primordial germ cell-like cells develop dynamically within integrated tissuesDevelopmentSeptember 1, 2023Christopher B. Cooke, Christopher Barrington, Peter Baillie-Benson, Jennifer Nichols, Naomi MorisPX459-Cas9 plasmid (Addgene, #62988) (Ran et al., 2013) after cleavage with BbsI (New England BioLabs, R0539S). The correct integration of the gRNAs was confirmed after cloning by Sanger sequencing using the hU6-F

(8) ATRX guards against aberrant differentiation in mesenchymal progenitor cellsbioRxivAugust 8, 2023Yan Fang, Douglas Barrows, Yakshi Dabas, Thomas S Carroll, William D. Tap, Benjamin A. Nacevgenerated using an empty vector without sgRNA but carrying Cas9. The vector was digested by Bbsl (NEB, R0539S) and dephosphorylated by calf intestinal alkaline phosphatase (NEB, M0290). The oligos were annealed

(9) Metabolic impact of genetic and chemical ADP/ATP carrier inhibition in renal proximal tubule epithelial cellsArchives of ToxicologyJuly 1, 2023Charlotte A. Hoogstraten, Maaike M. E. Jacobs, Guido de Boer, Melissa A. E. van de Wal, Werner J. H. Koopman, Jan A. M. Smeitink, Frans G. M. Russel, Tom J. J. SchirrisVerschuren, Dept. of Physiology, Radboudumc, Addgene, #64,073) was linearized at 37 °C for 1 h using BbsI (R0539, New England Biolabs, Ipswich, MA, USA), or BsaI (R0535, New England Biolabs) restriction enzymes. Ligation

(10) Epigenetic reprogramming-induced guanidinoacetic acid synthesis promotes pancreatic cancer metastasis and transcription-activating histone modificationsJournal of Experimental & Clinical Cancer ResearchJune 28, 2023Jinshou Yang, Bo Ren, Jie Ren, Gang Yang, Yuan Fang, Xing Wang, Feihan Zhou, Lei You, Yupei ZhaoP1715) with BbsI restriction enzymatic site. Briefly, 1 μg of plasmid was digested with BbsI (NEB, R0539S) at 37 ℃ for 30 min, and the digested plasmid was purified using the QIAquick Gel Extraction Kit and

(11) Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathyNature CommunicationsJune 22, 2023Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer, et al.annealed and ligated with a gRNA expression plasmid (Addgene ID: 53188), which was digested with BbsI (R0539S, NEB) prior to ligation. For stable base editor expression, the coding region of Cas9 from the lentiCRISPRv2

(12) eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome contentThe EMBO journalJune 15, 2023Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, et al.hydroperoxide,MACKLIN,Cat # 75‐91‐2 BsmBI,New England Biolabs,Cat # R0580S BbSI,New England Biolabs,Cat # R0539S XbaI,New England Biolabs,Cat # R0145S XhoI,New England Biolabs,Cat # R0146S BamHI,New England Biolabs,Cat

(13) Investigating pioneer factor activity and its coordination with chromatin remodelers using integrated synthetic oligo assaySTAR ProtocolsJune 7, 2023Hengye Chen, Chao Yan, Archana Dhasarathy, Michael Kladde, Lu BaiStates Biological,Cat# 37340-57-1 Ethylenediaminetetraacetic acid (EDTA),Sigma,Cat# EDS BbsI,NEB,Cat# R0539S BsaI,NEB,Cat# R3733S T4 DNA ligase,NEB,Cat# M0202S 10× NEB buffer 2.1,NEB,Cat# B6002S 10× NEB CutSmart buffer

(14) BESST: a novel LncRNA knockout strategy with less genome perturbanceNucleic acids researchMay 22, 2023Zhang S, Chen Y, Dong K, Zhao Y, Wang Y, Wang S, Qu C, Xu N, Xie W, Zeng C, et al.backbone (Addgene, #62988) (22). Briefly, the PX459 plasmid was linearized by BbsI (New England Biolabs, #R0539) and cleaned using the agarose gel DNA extraction kit (Takara, #9762). Subsequently, primers of each

(15) Transcription-Coupled Repair of DNA Interstrand Crosslinks by UVSSAbioRxivMay 13, 2023Rowyn Church Liebau, Crystal Waters, Arooba Ahmed, Rajesh K Soni, Jean Gautierab109411), RPA (Abcam ab2175).Restriction enzymes: all restriction enzymes acquired from NEB: BbsI (R0539S), BamHI (R0136S), NheI-(HF R3131S), BglII (R0144S), AscI (R0558S), EcoRI (R0101S)Kits: Q5 mutagenesis

(16) Membranous NECTIN-4 Expression Frequently Decreases during Metastatic Spread of Urothelial Carcinoma and Is Associated with Enfortumab Vedotin ResistanceClinical Cancer ResearchApril 14, 2023Niklas Klümper, Damian J. Ralser, Jörg Ellinger, Florian Roghmann, Julia Albrecht, Eduard Below, Abdullah Alajati, Danijel Sikic, Johannes Breyer, Christian Bolenz, et al.protocol (14). PX458 was digested with the restriction enzyme BbsI (New England Biolabs, catalog no: R0539L). Two different sgRNA sequences targeting the NECTIN4 gene were cloned into PX458. The following DNA

(17) In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cellsNature CommunicationsApril 14, 2023Maximilian Huber, Javier Casares-Arias, Reinhard Fässler, Daniel J. Müller, Nico Strohmeyer5’-phosphorylated forward and reverse DNA sequences encoding sgRNAs were hybridized, digested by Bbs1 (R0539S, New England Biolabs) and ligated into the plasmid pSpCas9-2A-BFP (px458.2)60, using T4 polymerase (M0203S,

(18) Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 CellsInternational journal of molecular sciencesApril 11, 2023Muranaka M, Takamatsu S, Ouchida T, Kanazawa Y, Kondo J, Nakagawa T, Egashira Y, Fukagawa K, Gu J, Okamoto T, et al.Vector The human VIP36-KO vector was prepared as follows. The purified products obtained from the BbsI (#R0539, New England Biolabs)-digested pX330-U6-Chimeric_BB-CBh-hSpCas9 vector (Plasmid #42230, Addgene, Watertown,

(19) Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cellsEngineering in Life SciencesApril 1, 2023Seul Mi Kim, Jaejin Lee, Jae Seong Leeplasmid vector using the USER enzyme (M5505L, New England Biolabs, Ipswich, MA) for USER cloning or Bbs1 (R0539L, NEB) and T4 DNA ligase (M0202L, NEB) for Golden Gate cloning. Two complementary oligonucleotides, including

(20) IFNγ is a central node of cancer immune equilibriumCell ReportsMarch 28, 2023Michael J. Walsh, Courtney T. Stump, Rakeeb Kureshi, Patrick Lenehan, Lestat R. Ali, Michael Dougan, David M. Knipe, Stephanie K. DouganIFNγ,Peprotech,315-05 Recombinant Human IL-2,Peprotech,200-02 XhoI,NEB,R0146S NotI,NEB,R0189S BbsI,NEB,R0539S SwaI,NEB,R0604S BsmBI-v2,NEB,R0739S T4 DNA ligase,NEB,M0202S Puromycin Dihydrochloride,ThermoFisher,A1113803 Hygromycin B (50 mg/mL

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BbsI

BbsI from New England Biolabs

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New England Biolabs

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BbsI from New England Biolabs

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